Application

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Chd2 from C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Chd2 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Chd2 from C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

This Anti-Chd2 antibody, clone 8H3 is validated for use in western blotting, IP, ICC & ChIP for the detection of Chd2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Chd2, also known as Chromodomain-helicase-DNA-binding protein 2 (CHD-2), or ATP-dependent helicase CHD2, and encoded by the gene CHD2, belongs to the CHD family of proteins. This family is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes are chromatin remodeling proteins and alter gene expression by modifying the chromatin structure of a particular gene. The alteration affects the access of the transcriptional apparatus to it, and thus the gene’s expression. In the mouse Chd2 is expressed in heart, forebrain, extremities, and facial and dorsal regions during specific times of embryonic development. Chd2 null mice are embryonic lethal, and in humans, various mutations in CHD-2 gene appear to cause a variety of disorders and developmental defects. Other mutations associated with the CHD2 gene locus appear to cause childhood-onset epileptic encephalopathy (EEOC).

Immunogen

Recombinant protein corresponding to mouse Chd2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Chd2 in 10 µg of Jurkat cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~211 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. Two known isoforms may be observed at 50 kDa and 200 kDa